6KRU

Crystal structure of mouse IgG2b Fc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	90
Detector technology	CCD
Collection date	2015-07-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9000
Spacegroup name	P 21 21 21
Unit cell lengths	112.550, 130.165, 134.189
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 2.300
R-factor	0.2001
Rwork	0.198
R-free	0.23670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rgs
RMSD bond length	0.010
RMSD bond angle	1.188
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.063	1.015
Number of reflections	88009	4370
<I/σ(I)>	18.3	1.8
Completeness [%]	99.9	97.9
Redundancy	7.4	7.5
CC(1/2)	0.999	0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	16% PEG3350, 0.2 M lithium chloride, and 0.1 M Bis-Tris (pH 6.5)