6KR6
Crystal structure of Drosophila Piwi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-15 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.090, 115.640, 119.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.900 - 2.900 |
R-factor | 0.2404 |
Rwork | 0.239 |
R-free | 0.25850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5guh |
RMSD bond length | 0.002 |
RMSD bond angle | 0.566 |
Data reduction software | DIALS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.930 | 39.930 | 3.080 |
High resolution limit [Å] | 2.900 | 8.520 | 2.900 |
Rmerge | 0.548 | 0.194 | 21.100 |
Rmeas | 0.551 | 0.196 | 21.241 |
Number of reflections | 36974 | 1445 | 5991 |
<I/σ(I)> | 10.12 | 44.58 | 0.52 |
Completeness [%] | 100.0 | 99 | 100 |
Redundancy | 74.447 | 79.409 | 75.512 |
CC(1/2) | 0.998 | 0.998 | 0.676 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 20 % PEG 6000, 0.1 M MES pH6.0, and 0.01 M zinc chloride |