6KR5
Crystal structure of O-Acetyl Serine Sulfhydrylase isoform 3 from Entamoeba histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 193.15 |
| Detector technology | CCD |
| Collection date | 2016-02-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.505, 85.874, 91.164 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.366 - 1.544 |
| R-factor | 0.1809 |
| Rwork | 0.180 |
| R-free | 0.21470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pqm |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_3352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.540 | 1.550 |
| Rmeas | 0.074 | 0.920 |
| Rpim | 0.270 | |
| Number of reflections | 92112 | 4544 |
| <I/σ(I)> | 44 | 2 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 7.3 | 7 |
| CC(1/2) | 0.830 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289.15 | 0.1M PCTP buffer, 25% PEG 1500 |
