6KR1
ATP dependent protease HslV from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-03-28 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 32 |
| Unit cell lengths | 94.304, 94.304, 227.374 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.160 - 2.000 |
| R-factor | 0.2187 |
| Rwork | 0.216 |
| R-free | 0.26590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yyf |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.378 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.082 | 0.526 |
| Rpim | 0.028 | 0.260 |
| Number of reflections | 150435 | 7367 |
| <I/σ(I)> | 17.6 | 2.2 |
| Completeness [%] | 98.6 | 96.6 |
| Redundancy | 6.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | HEPES:NaOH, Lithium Sulfate |
