6KQR

A pre-assembled molecular-helical Cascade backbone of Csy3 subunits from Zymomonas mobilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-05
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	82.181, 116.163, 115.937
Unit cell angles	99.37, 103.12, 103.35

Refinement procedure

Resolution	14.996 - 2.899
R-factor	0.2161
Rwork	0.214
R-free	0.26720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uz9
RMSD bond length	0.010
RMSD bond angle	1.218
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.950
High resolution limit [Å]	2.899	2.899
Number of reflections	86087	4309
<I/σ(I)>	16
Completeness [%]	98.4
Redundancy	1.9
CC(1/2)	0.942	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1M Bis-Tris pH 6.5, 2.0M ammonium sulfate