6KO7
Crystal structure of the Ethidium bound RamR determined with XtaLAB Synergy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-15 |
| Detector | RIGAKU HyPic-6000HE |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.164, 54.276, 91.412 |
| Unit cell angles | 103.42, 97.45, 90.14 |
Refinement procedure
| Resolution | 23.450 - 1.700 |
| R-factor | 0.1718 |
| Rwork | 0.169 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vvy |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.845 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless (0.5.29) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.450 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.069 | 0.402 |
| Rmeas | 0.084 | 0.548 |
| Rpim | 0.047 | 0.371 |
| Number of reflections | 88793 | 4303 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 98.7 | 94.8 |
| Redundancy | 2.4 | 1.8 |
| CC(1/2) | 0.994 | 0.479 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | PEG3350, ammonium sulfate, sodium Citrate |
