6KM9
Crystal structure of SucA from Vibrio vulnificus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.271, 76.708, 144.579 |
Unit cell angles | 90.00, 100.56, 90.00 |
Refinement procedure
Resolution | 47.377 - 2.724 |
R-factor | 0.1941 |
Rwork | 0.192 |
R-free | 0.24920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jgd |
RMSD bond length | 0.006 |
RMSD bond angle | 0.859 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.720 | 2.750 |
Rpim | 0.120 | 0.424 |
Number of reflections | 47954 | 2401 |
<I/σ(I)> | 6.4 | |
Completeness [%] | 98.7 | |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 220 mM calcium acetate, 100 mM 2-(Bis(2-hydroxyethyl)amino)acetic acid (BICINE) (pH 9.0), 50% (v/v) PEG 300, 0.1 mM ThDP, and 0.1 mM MgCl2 |