6KK1
Structure of thermal-stabilised(M8) human GLP-1 receptor transmembrane domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2017-01-17 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.930, 67.350, 83.680 |
| Unit cell angles | 91.07, 90.10, 107.96 |
Refinement procedure
| Resolution | 49.504 - 2.800 |
| R-factor | 0.2458 |
| Rwork | 0.244 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vew |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.441 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.500 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.130 | 0.400 |
| Number of reflections | 25859 | 3446 |
| <I/σ(I)> | 5.3 | 2.2 |
| Completeness [%] | 77.9 | 70.9 |
| Redundancy | 3.1 | 2.6 |
| CC(1/2) | 0.980 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 0.4-0.45 M ammonium acetate, 0.1 M sodium cacodylate, pH 6.2-6.6, 35-38% PEG400, 3% w/v aminohexanoic acid |
