6KJ9
E. coli ATCase catalytic subunit mutant - G128/130A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS IV |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-09-23 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.758, 96.729, 121.694 |
| Unit cell angles | 90.00, 94.16, 90.00 |
Refinement procedure
| Resolution | 48.360 - 2.500 |
| R-factor | 0.1928 |
| Rwork | 0.192 |
| R-free | 0.22830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1za1 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 65582 | 3280 |
| <I/σ(I)> | 18.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.994 | 0.784 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M NH4Ac, 0.1M Tris pH 8.5, 20% PEG3350, and 10% glycerol |
