6KHY
The crystal structure of AsfvAP:AG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2018-12-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 110.512, 148.504, 178.058 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.934 - 3.008 |
| R-factor | 0.2125 |
| Rwork | 0.211 |
| R-free | 0.24920 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.554 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL2Map |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.440 | 3.000 |
| Rmerge | 0.085 | 0.043 | 0.362 |
| Rmeas | 0.091 | 0.045 | 0.405 |
| Rpim | 0.031 | 0.013 | 0.175 |
| Total number of observations | 215889 | ||
| Number of reflections | 28960 | 3070 | 2771 |
| <I/σ(I)> | 6.7 | ||
| Completeness [%] | 98.3 | 99.1 | 96 |
| Redundancy | 7.5 | 10.7 | 4.5 |
| CC(1/2) | 0.999 | 0.621 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 16% w/v PEG 3350 0.1 M Potassium sodium tartrate tetrahydrate |
