6KHY
The crystal structure of AsfvAP:AG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2018-12-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 110.512, 148.504, 178.058 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.934 - 3.008 |
R-factor | 0.2125 |
Rwork | 0.211 |
R-free | 0.24920 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.554 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL2Map |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.440 | 3.000 |
Rmerge | 0.085 | 0.043 | 0.362 |
Rmeas | 0.091 | 0.045 | 0.405 |
Rpim | 0.031 | 0.013 | 0.175 |
Total number of observations | 215889 | ||
Number of reflections | 28960 | 3070 | 2771 |
<I/σ(I)> | 6.7 | ||
Completeness [%] | 98.3 | 99.1 | 96 |
Redundancy | 7.5 | 10.7 | 4.5 |
CC(1/2) | 0.999 | 0.621 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 16% w/v PEG 3350 0.1 M Potassium sodium tartrate tetrahydrate |