6KEZ

Crystal structure of GAPDH/CP12/PRK complex from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	92.813, 204.734, 157.036
Unit cell angles	90.00, 106.88, 90.00

Refinement procedure

Resolution	50.000 - 3.500
Rwork	0.246
R-free	0.28680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rvd 6kew
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.560
High resolution limit [Å]	3.500	3.500
Rmerge	0.123	0.554
Number of reflections	68615	3452
<I/σ(I)>	7.56
Completeness [%]	97.8
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	100mM HEPES, pH7.5, 100mM KCl, 15% polyethylene glycol 6000