6KEO
Crystal structure of Drosophila melanogaster Noppera-bo, glutathione S-transferase epsilon 14 (DmGSTE14), in 17beta-estradiol-bound form
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.398, 75.080, 109.015 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.490 - 1.700 |
| R-factor | 0.1767 |
| Rwork | 0.175 |
| R-free | 0.21130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kem |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.964 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.490 | 1.636 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.048 | 0.840 |
| Rmeas | 0.050 | 0.883 |
| Number of reflections | 57956 | 3057 |
| <I/σ(I)> | 37.59 | 2.61 |
| Completeness [%] | 87.3 | 46.64 |
| Redundancy | 14.1 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 42.5%(v/v) PPG400, 100mM Bis-Tris, and pH 6.4 |
