6KDT
Crystal structure of human DNMT3B (Q772R)-DNMT3L complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2019-02-28 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 60.350, 240.784, 232.131 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.370 - 2.870 |
Rwork | 0.194 |
R-free | 0.24220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yx2 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14-3260-000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.127 | |
Number of reflections | 36776 | 2796 |
<I/σ(I)> | 13.24 | |
Completeness [%] | 97.1 | |
Redundancy | 5.2 | |
CC(1/2) | 0.824 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 278 | 2.2 M sodium formate |