6KDA

Crystal structure of human DNMT3B-DNMT3L in complex with DNA containing CpGpG site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	110
Detector technology	CCD
Collection date	2018-11-01
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 31
Unit cell lengths	193.872, 193.872, 49.741
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.500 - 2.909
R-factor	0.1892
Rwork	0.188
R-free	0.21990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yx2
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.010
High resolution limit [Å]	2.909	2.910
Rmerge	0.061	0.745
Number of reflections	42182	2208
<I/σ(I)>	25.95	2.05
Completeness [%]	100.0	99.8
Redundancy	4.8
CC(1/2)	0.972	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	0 mM MES (pH 5.7), 160 mM KCl, 10 mM MgSO4 and 14% PEG 400