6KC5
HOIP-HOIPIN1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.350, 60.227, 92.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.140 - 1.543 |
R-factor | 0.1991 |
Rwork | 0.198 |
R-free | 0.21690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ljq |
RMSD bond length | 0.008 |
RMSD bond angle | 1.445 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.640 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.062 | 1.580 |
Number of reflections | 32942 | 5152 |
<I/σ(I)> | 18.22 | |
Completeness [%] | 99.6 | 97.8 |
Redundancy | 8.68 | 6.39 |
CC(1/2) | 0.999 | 0.745 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100 mM HEPES-Na (pH 7.5), 200 mM NaCl, 25% PEG 3,350 |