6KBN

Crystal structure of Vac8 (del 19-33) bound to Atg13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	69.475, 85.268, 272.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.113 - 3.200
R-factor	0.2182
Rwork	0.216
R-free	0.25460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xjg
RMSD bond length	0.008
RMSD bond angle	1.667
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Rmerge	0.103	0.537
Number of reflections	27903	1320
<I/σ(I)>	17.88
Completeness [%]	98.7
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 3350, N-(2-Acetamido)iminodiacetic acid, Ammonium sulfate