6KAG

Crystal structure of the SMARCB1/SMARCC2 subcomplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9778
Spacegroup name	P 41 3 2
Unit cell lengths	170.390, 170.390, 170.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.187 - 2.601
R-factor	0.2083
Rwork	0.207
R-free	0.24020
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.002
Data reduction software	HKL-2000 (714)
Data scaling software	HKL-2000 (714)
Phasing software	AutoSol ((1.10.1_2155: 000))
Refinement software	PHENIX ((1.10.1_2155: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rpim	0.044	0.279
Number of reflections	26616	2610
<I/σ(I)>	17.13
Completeness [%]	100.0
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	150 mM CsCl2, 15% PEG3350.