6K9A
The complex of NrS-1 N terminal domain (1-305) with dGTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 77.15 |
Detector technology | CCD |
Collection date | 2018-05-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 119.114, 58.007, 47.135 |
Unit cell angles | 90.00, 92.96, 90.00 |
Refinement procedure
Resolution | 29.739 - 2.300 |
R-factor | 0.2112 |
Rwork | 0.209 |
R-free | 0.24770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.530 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.119 | 0.311 |
Rmeas | 0.133 | 0.367 |
Number of reflections | 14335 | 1390 |
<I/σ(I)> | 8.7 | 2.4 |
Completeness [%] | 98.4 | 95.7 |
Redundancy | 4.6 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291.15 | 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% w/v Polyethylene glycol 4K |