6K8N
Crystal structure of the Sulfolobus solfataricus topoisomerase III
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 110.671, 90.040, 156.184 |
Unit cell angles | 90.00, 100.52, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.205 |
Rwork | 0.203 |
R-free | 0.24690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gaj |
RMSD bond length | 0.008 |
RMSD bond angle | 0.885 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2747: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.057 | 0.942 |
Number of reflections | 172718 | 8284 |
<I/σ(I)> | 25.8 | 2 |
Completeness [%] | 98.0 | 94.5 |
Redundancy | 7 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 285 | 8% (v/v) Tacsimate (pH 4.4), and 13.75% (w/v) Polyethyleneglycerol 3,350 |