6K8K

Crystal structure of Arabidopsis thaliana BIC2-CRY2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97919
Spacegroup name	P 65 2 2
Unit cell lengths	142.552, 142.552, 526.434
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.425 - 2.500
R-factor	0.1733
Rwork	0.171
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u3c
RMSD bond length	0.008
RMSD bond angle	0.989
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.101	0.784
Rmeas	0.104	0.807
Rpim	0.024	0.186
Number of reflections	109254	5043
<I/σ(I)>	21.3	4.4
Completeness [%]	99.2	94.6
Redundancy	19.2
CC(1/2)	0.999	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	Tris, Calcium acetate hydrate, PEG 3350