6K81

Crystal structure of human VASH1-SVBP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	70.452, 128.995, 44.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.650 - 2.280
R-factor	0.1922
Rwork	0.190
R-free	0.24190
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.580
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	PHENIX ((1.15_3459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.360
High resolution limit [Å]	2.280	2.280
Number of reflections	18798	1579
<I/σ(I)>	30.1
Completeness [%]	97.8
Redundancy	11.1
CC(1/2)	0.996	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	Calcium acetate, PEG 3000