6K7T

Crystal structure of bat (Pteropus Alecto) MHC class I Ptal-N*01:01 in complex with Hendra virus-derived peptide HeV1--human beta-2 microglobulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	OSCILLATION CAMERA
Collection date	2017-01-14
Detector	SDMS
Wavelength(s)	0.97915
Spacegroup name	P 21 21 2
Unit cell lengths	73.879, 88.766, 67.174
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.600
Rwork	0.211
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zuw
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.070	0.070
Number of reflections	58687	58687
<I/σ(I)>	27.422
Completeness [%]	99.5
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M HEPES pH 7.0, 2% w/v Polyethylene glycol 3350