6K6B

Application of anti-helix antibodies in protein structure determination (8496-3LRH)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	103
Detector technology	CCD
Collection date	2016-06-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	52.559, 51.351, 55.464
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.279 - 2.060
R-factor	0.2057
Rwork	0.202
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lrh 1dee
RMSD bond length	0.002
RMSD bond angle	0.412
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2142: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.000	2.000
Rpim	0.035	0.190
Number of reflections	9479	1369
<I/σ(I)>	25.2
Completeness [%]	99.0
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	35% PEG 4000, 0.1M MES pH 6.5