6K68
Application of anti-helix antibodies in protein structure determination (8420-3MNZ)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2015-11-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.951, 95.017, 179.561 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.420 - 3.200 |
R-factor | 0.2913 |
Rwork | 0.289 |
R-free | 0.31840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mnz 1dee |
RMSD bond length | 0.002 |
RMSD bond angle | 0.512 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.300 |
High resolution limit [Å] | 3.200 | 3.200 |
Rpim | 0.109 | 0.346 |
Number of reflections | 22763 | 22763 |
<I/σ(I)> | 7.4 | |
Completeness [%] | 98.3 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | 20% PEG MME 2000, 0.1M MOPS pH 6.5 |