6K65

Application of anti-helix antibodies in protein structure determination (9014-1P4B)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	103
Detector technology	CCD
Collection date	2016-09-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	35.620, 120.181, 84.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.217 - 1.650
R-factor	0.1708
Rwork	0.170
R-free	0.19180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p4b 1dee
RMSD bond length	0.008
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.700
High resolution limit [Å]	1.600	1.600
Rpim	0.032	0.220
Number of reflections	44481	4320
<I/σ(I)>	31.8
Completeness [%]	99.5
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	1.8M NaK phosphate, 0.1M HEPES pH 7.5,