6K64
Application of anti-helix antibodies in protein structure determination (8188-3LRH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2016-05-12 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.061, 41.261, 62.436 |
| Unit cell angles | 85.97, 75.49, 68.26 |
Refinement procedure
| Resolution | 30.439 - 1.933 |
| R-factor | 0.1742 |
| Rwork | 0.171 |
| R-free | 0.20680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lrh 1dee |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.730 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2142: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.032 | 0.111 |
| Number of reflections | 22184 | 1830 |
| <I/σ(I)> | 28 | |
| Completeness [%] | 95.5 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 35% PEG 4000 0.1M MOPS pH 6.5 |
