6K63
The crystal structure of cytidine deaminase from Klebsiella pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-04-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979617 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 151.748, 151.748, 120.155 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.904 - 2.073 |
| R-factor | 0.1758 |
| Rwork | 0.175 |
| R-free | 0.22280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ctu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.802 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Number of reflections | 96550 | 9 |
| <I/σ(I)> | 31 | 7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.1 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 0.1 M HEPES(pH 5.5) 1.72 M Ammonium sulfate 7% v/v 1,4-Dioxane |
