6K3M
Application of anti-helix antibodies in protein structure determination (8189-3LRH)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-12 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 27.345, 56.102, 96.096 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.000 - 1.800 |
R-factor | 0.19179 |
Rwork | 0.190 |
R-free | 0.23123 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lrh 1dee |
RMSD bond length | 0.011 |
RMSD bond angle | 1.710 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rpim | 0.022 | 0.037 |
Number of reflections | 14114 | 14114 |
<I/σ(I)> | 39.1 | |
Completeness [%] | 98.4 | |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | 30% PEG 4000, 0.2M MgCl2, 0.1M MOPS pH 6.5 |