6K39

Structural analysis of AIMP2-DX2 and HSP70 interaction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-02
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97930
Spacegroup name	P 21 21 2
Unit cell lengths	56.111, 111.581, 45.571
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.632 - 1.398
R-factor	0.168173872142
Rwork	0.167
R-free	0.19181
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jpv
RMSD bond length	0.007
RMSD bond angle	0.897
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.12_2829)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.632	1.448
High resolution limit [Å]	1.398	1.398
Rmerge	0.092	0.776
Rmeas	0.099	0.835
Rpim	0.035	0.304
Number of reflections	57251	5431
<I/σ(I)>	15.56	4.81
Completeness [%]	99.5	95.65
Redundancy	7.8	7.3
CC(1/2)	0.997	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	PEG1500, Succinic Acid, Sodium Dihydrogen Phosphate, Glycine