6K1T
The structure of Francisella virulence factor BioJ
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.943, 67.313, 55.571 |
Unit cell angles | 90.00, 112.55, 90.00 |
Refinement procedure
Resolution | 40.208 - 1.584 |
R-factor | 0.2082 |
Rwork | 0.207 |
R-free | 0.23500 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.808 |
Data scaling software | autoPROC |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.320 | 1.670 |
High resolution limit [Å] | 1.580 | 1.580 |
Number of reflections | 37190 | 5609 |
<I/σ(I)> | 6.7 | |
Completeness [%] | 91.5 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | COUNTER-DIFFUSION | 289.15 | 20% (v/v) PEG 6000, 100mM BICINE/sodium hydroxide (pH 9.0) |