6K1N
PLP-bound form of a putative cystathionine gamma-lyase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.025, 141.735, 157.833 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 105.455 - 2.260 |
R-factor | 0.1896 |
Rwork | 0.188 |
R-free | 0.24230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nmp |
RMSD bond length | 0.008 |
RMSD bond angle | 1.038 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 157.830 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 93522 | 2374 |
<I/σ(I)> | 5.6 | |
Completeness [%] | 97.5 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 289 | 26% (v/v) polyethylene glycol 400, 0.2 M Calcium, 0.1 mM Na Acetate pH 4.2, 2 mM PLP |