6JZD

Crystal structure of peptide-bound VASH2-SVBP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	74.004, 111.339, 126.317
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.110 - 2.479
R-factor	0.1816
Rwork	0.179
R-free	0.22960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.943
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14rc3_3206: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.580
High resolution limit [Å]	2.450	2.480
Rmerge	0.059	0.759
Rpim	0.025	0.332
Number of reflections	18801	2048
<I/σ(I)>	17.1
Completeness [%]	99.6	98.5
Redundancy	6.4	6.1
CC(1/2)	0.999	0.764

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291.15	HEPES, potassium chloride, Ammonium sulphate