6JXM
Crystal Structure of phi29 pRNA domain II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-17 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.606 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 120.178, 120.178, 187.494 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 101.180 - 3.320 |
R-factor | 0.22702 |
Rwork | 0.223 |
R-free | 0.26515 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mgm |
RMSD bond length | 0.006 |
RMSD bond angle | 1.209 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 101.180 | 3.590 |
High resolution limit [Å] | 3.320 | 3.320 |
Rmerge | 0.067 | |
Rpim | 0.041 | 0.029 |
Number of reflections | 10506 | |
<I/σ(I)> | 13.9 | |
Completeness [%] | 99.9 | |
Redundancy | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.12 mM spermine, 5 mM cobalt hexamine, 10%(+/-)-2-methyl-2,4-pentanediol (MPD), 62 mM potassium chloride, 30 mM barium chloride, 40 mM pH 6 Na cacodylate |