6JXF
Photoswitchable fluorescent protein Gamillus, off-state (pH7.0)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-18 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.900 |
Spacegroup name | I 21 3 |
Unit cell lengths | 161.711, 161.711, 161.711 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.680 - 1.800 |
R-factor | 0.1543 |
Rwork | 0.154 |
R-free | 0.16770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5y00 |
RMSD bond length | 0.034 |
RMSD bond angle | 2.939 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.682 | 46.682 | 1.910 |
High resolution limit [Å] | 1.800 | 5.360 | 1.800 |
Rmerge | 0.064 | 0.035 | 0.438 |
Rmeas | 0.069 | 0.038 | 0.470 |
Total number of observations | 480868 | ||
Number of reflections | 64910 | 2452 | 10396 |
<I/σ(I)> | 17.34 | 35.06 | 3.91 |
Completeness [%] | 99.7 | 95.1 | 99.5 |
Redundancy | 7.408 | 6.714 | 7.55 |
CC(1/2) | 0.999 | 0.999 | 0.939 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | Ammonium phosphate, sodium citrate, sodium chloride, HEPES, pH5.5 |