6JWC

Mouse antibody 2B5 Fab in complex with PEG

Experimental procedure

Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-05
Detector	RAYONIX MX300HE
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	98.895, 98.895, 96.711
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.886 - 2.297
R-factor	0.1889
Rwork	0.187
R-free	0.22550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ju0
RMSD bond length	0.002
RMSD bond angle	0.613
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rpim	0.030	0.296
Number of reflections	21952	2123
<I/σ(I)>	25.3	2.8
Completeness [%]	99.9	100
Redundancy	7	7
CC(1/2)	0.954	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1