6JVB
Crystal Structure of Human CRMP2 1-532, AGE-modified
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-21 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.206, 147.329, 95.233 |
| Unit cell angles | 90.00, 103.91, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.18438 |
| Rwork | 0.183 |
| R-free | 0.20371 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mkv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.387 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.104 | 0.622 |
| Rpim | 0.062 | 0.363 |
| Number of reflections | 171234 | 8561 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.9 |
| CC(1/2) | 0.980 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | 0.1 M MES (pH 6.0), 15% (v/v) PEG 400, 0.1M calcium acetate |
