6JUZ
Crystal Structure of N-terminal domain of ArgZ(N71S) covalently bond to a reaction intermediate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97916 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.530, 87.549, 43.196 |
Unit cell angles | 90.00, 93.74, 90.00 |
Refinement procedure
Resolution | 25.026 - 1.210 |
R-factor | 0.1661 |
Rwork | 0.166 |
R-free | 0.17540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jv0 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.230 |
High resolution limit [Å] | 1.210 | 3.280 | 1.210 |
Rmerge | 0.054 | 0.042 | 0.489 |
Rmeas | 0.061 | 0.048 | 0.564 |
Rpim | 0.027 | 0.022 | 0.276 |
Number of reflections | 89713 | 4435 | 4456 |
<I/σ(I)> | 18.3 | ||
Completeness [%] | 98.2 | 95.1 | 97.2 |
Redundancy | 4.8 | 4.8 | 3.8 |
CC(1/2) | 0.993 | 0.804 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.2 M KI, 0.1 M MES pH 6.5, 19% PEG 4000 |