6JRP
Crystal structure of CIC-HMG-ETV5-DNA complex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-05 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.9778, 0.9785 |
Spacegroup name | P 32 |
Unit cell lengths | 66.082, 66.082, 158.285 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.791 - 3.000 |
R-factor | 0.3006 |
Rwork | 0.297 |
R-free | 0.33430 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.067 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.109 | 0.704 |
Number of reflections | 15474 | 727 |
<I/σ(I)> | 26.56 | |
Completeness [%] | 100.0 | |
Redundancy | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium acetate, PEG3350, Bis-tris pH 6.5 |