6JPV

Structural analysis of AIMP2-DX2 and HSP70 interaction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	41.111, 77.054, 88.711
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.079 - 2.150
R-factor	0.17847200903
Rwork	0.174
R-free	0.23479
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wv5
RMSD bond length	0.007
RMSD bond angle	0.958
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.12_2829)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.080	2.227
High resolution limit [Å]	2.150	2.150
Rmerge	0.104	0.235
Rmeas	0.112	0.252
Rpim	0.041	0.090
Number of reflections	15918	1551
<I/σ(I)>	14.26	8.73
Completeness [%]	99.7	99.87
Redundancy	7.7	8
CC(1/2)	0.994	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	PEG1500, Succinic Acid, Sodium Dihydrogen Phosphate, Glycine