6JPR
Crystal structure of Phycocyanin from Nostoc sp. R76DM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.136, 186.158, 85.532 |
| Unit cell angles | 90.00, 94.33, 90.00 |
Refinement procedure
| Resolution | 37.260 - 2.350 |
| R-factor | 0.2014 |
| Rwork | 0.199 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bv8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.096 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.540 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.090 | 0.406 |
| Number of reflections | 84711 | 4592 |
| <I/σ(I)> | 8.5 | 2.5 |
| Completeness [%] | 97.7 | 99 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | PEG 3350, sodium malonate |
