6JON
Crystal structures of phage NrS-1 N300-dNTPs-Mg2+ complex provide molecular mechanisms for substrate specificity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97914 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.820, 55.290, 50.550 |
| Unit cell angles | 90.00, 96.06, 90.00 |
Refinement procedure
| Resolution | 36.584 - 2.340 |
| R-factor | 0.2238 |
| Rwork | 0.223 |
| R-free | 0.23340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6a9w |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.584 | 2.400 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.109 | 1.252 |
| Number of reflections | 29520 | 8011 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 97.3 | 96.4 |
| Redundancy | 6.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 100mM Bis-Tris, pH 6.5, 0.2M Li2SO4, 25% PEG 3350 |
