6JNK

Crystal structure of Azospirillum brasilense L-arabinose 1-dehydrogenase (NADP-bound form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-04
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	70.745, 97.526, 104.762
Unit cell angles	90.00, 104.15, 90.00

Refinement procedure

Resolution	46.636 - 2.200
R-factor	0.2117
Rwork	0.209
R-free	0.26760
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.200	2.200
Rmeas	0.155	1.015
Number of reflections	68572	22054
<I/σ(I)>	6.26
Completeness [%]	99.1	97.8
Redundancy	3.53	3.62
CC(1/2)	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	25% PEG 3350, 0.1 M Tris-HCl pH 8.5, 0.2 M NaCl