6JM4

The crystal structure of PB1 homo-dimer of human P62/SQSTM1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97943
Spacegroup name	C 1 2 1
Unit cell lengths	112.483, 53.517, 61.457
Unit cell angles	90.00, 94.29, 90.00

Refinement procedure

Resolution	48.301 - 3.200
R-factor	0.242593207011
Rwork	0.239
R-free	0.27620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2kkc
RMSD bond length	0.002
RMSD bond angle	0.487
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.301	3.314
High resolution limit [Å]	3.200	3.200
Rmerge	0.081	0.262
Number of reflections	5366
<I/σ(I)>	10.54
Completeness [%]	87.0
Redundancy	1.1
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	1.8 M DL-Malic acid (pH 7.0), 0.1 M Tris-HCl (pH 7.0)