6JKI
Crystal structure and catalytic mechanism of the essential m1G37 tRNA methyltransferase TrmD from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2019-01-31 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.890575 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 85.670, 85.670, 147.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.730 - 2.590 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gm8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.190 | 2.730 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmeas | 0.192 | |
| Number of reflections | 37629 | 6010 |
| <I/σ(I)> | 14.38 | |
| Completeness [%] | 99.8 | |
| Redundancy | 19.6 | |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293.15 | 0.1M Tris-HCl, pH 8.4, 12.5% (v/v) MPD, 12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, 5% (w/v) PEG 200 |
