6JHW

Structure of anti-CRISPR AcrIIC3 and NmeCas9 HNH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298
Detector technology	PIXEL
Collection date	2018-07-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97940
Spacegroup name	P 43
Unit cell lengths	53.821, 53.821, 163.363
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.040
R-factor	0.22043
Rwork	0.219
R-free	0.23925
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jhv
RMSD bond length	0.013
RMSD bond angle	1.581
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.080
High resolution limit [Å]	2.040	2.040
Rmerge	0.071	0.413
Number of reflections	29510	1526
<I/σ(I)>	36.2	3.6
Completeness [%]	99.6	100
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	298	0.07 M NaCl, 0.05 M sodium citrate, pH 4.5, 22% (v/v) PEG 400