6JHW
Structure of anti-CRISPR AcrIIC3 and NmeCas9 HNH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2018-07-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97940 |
Spacegroup name | P 43 |
Unit cell lengths | 53.821, 53.821, 163.363 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.040 |
R-factor | 0.22043 |
Rwork | 0.219 |
R-free | 0.23925 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jhv |
RMSD bond length | 0.013 |
RMSD bond angle | 1.581 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.080 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.071 | 0.413 |
Number of reflections | 29510 | 1526 |
<I/σ(I)> | 36.2 | 3.6 |
Completeness [%] | 99.6 | 100 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 0.07 M NaCl, 0.05 M sodium citrate, pH 4.5, 22% (v/v) PEG 400 |