6JHP

Crystal structure of the glycoside hydrolase family 36 alpha-galactosidase from Paecilomyces thermophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	103.250, 173.043, 177.277
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.784 - 2.559
R-factor	0.1696
Rwork	0.167
R-free	0.22630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fnq
RMSD bond length	0.009
RMSD bond angle	1.010
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.784	2.650
High resolution limit [Å]	2.559	2.559
Rmerge	0.886	0.634
Number of reflections	94877	6607
<I/σ(I)>	5.87
Completeness [%]	92.2
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	293	1.8 M monosodium phosphate-dipotassium phosphate, pH 8.2