6JDX
Crystal structure of AcrIIC2 dimer in complex with partial Nme1Cas9 preprocessed with protease alpha-Chymotrypsin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-28 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97894 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.374, 77.236, 107.601 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.003 - 2.280 |
R-factor | 0.2087 |
Rwork | 0.208 |
R-free | 0.22240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jd7 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.343 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3247: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.320 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.087 | 1.071 |
Rmeas | 0.091 | 1.124 |
Rpim | 0.027 | 0.335 |
Number of reflections | 21775 | 1073 |
<I/σ(I)> | 26.6 | 2.3 |
Completeness [%] | 99.1 | 99.9 |
Redundancy | 10.8 | 10.7 |
CC(1/2) | 0.915 | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.9 | 289 | 0.1M HEPES pH 7.9, 14% PEG 20000 |