6JDW

CRYSTAL STRUCTURE OF HUMAN L-ARGININE:GLYCINE AMIDINOTRANSFERASE IN COMPLEX WITH GAMMA-AMINO BUTYRIC ACID

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	83.620, 83.620, 200.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.164
Rwork	0.164
Structure solution method	OTHER
RMSD bond length	0.010
RMSD bond angle	1.560
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.500 *
Rmerge	0.082 *	0.350 *
Total number of observations	73220 *
Number of reflections	22203 *
Completeness [%]	90.7 *	90.7 *
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		drop was made of a 7 micro litter protein solution and 14 micro litter of a reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	13 (mg/ml)
2	1	drop	HEPES-NaOH	30 (mM)
3	1	drop	EDTA	0.5 (mM)
4	1	drop	GSH	0.5 (mM)
5	1	reservoir	PEG6000	3 (%(w/v))
6	1	reservoir	HEPES-NaOH	40 (mM)