6JCK

Complex structure of Axin-DIX and Dvl2-DIX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-31
Detector	RAYONIX MX300HE
Wavelength(s)	0.9000
Spacegroup name	P 21 21 2
Unit cell lengths	49.000, 56.700, 60.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.600 - 3.090
R-factor	0.258
Rwork	0.256
R-free	0.28990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wsp 4wip
RMSD bond length	0.003
RMSD bond angle	0.732
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3235)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.170
High resolution limit [Å]	3.090	3.090
Rmeas	0.266	1.540
Number of reflections	3290	231
<I/σ(I)>	5.31	0.94
Completeness [%]	97.7	95.9
Redundancy	3.9	3.69
CC(1/2)	0.985	0.575

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	25% (w/v) PEG1500, 0.1 M Malonate-Imidazole-Borate buffer pH 7.0