6JBG

Crystal structure of the bacterial ribosomal decoding site in complex with 4'-deoxy-4'-fluoro neamine analog (equatorial 4'-F)

Replaces:  5BXK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-09
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	28.382, 83.720, 51.444
Unit cell angles	90.00, 95.10, 90.00

Refinement procedure

Resolution	43.704 - 3.100
R-factor	0.1667
Rwork	0.161
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j7t
RMSD bond length	0.010
RMSD bond angle	0.953
Data scaling software	XSCALE
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.704	43.704	3.180
High resolution limit [Å]	3.100	13.860	3.100
Rmerge	0.128	0.041	0.363
Rmeas	0.139	0.045	0.393
Number of reflections	4396	52	333
<I/σ(I)>	11.19	29.93	3.56
Completeness [%]	99.8	98.1	97.7
Redundancy	6.69	5.846	6.79
CC(1/2)	0.998	0.999	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, KCl, Sodium cacodylate, Spermine tetrahydrochloride